PATHWAY PROFILING ANTIBODIES & REAGENTS

     
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Imgenex Vectors
1. IMG-700 plasmid information

IMG-700 is a plasmid-based system to generate siRNAs. Inserts cloned into this vector express RNAs under U6 promoter in transfected mammalian cells.

2. IMG-1200 plasmid information

The adenoviral siRNA system allows viral-mediated delivery of siRNA into various cell types particularly that are not amenable to efficient transfection. The ability of viral vectors to transduce cells efficiently in vitro and in vivo, coupled with the efficacy of virally expressed siRNA, extends the application of siRNA to viral-based therapies and in vivo targeting experiments. IMG-1200 siRNA system is based on the expression of short hairpin RNAs from a modified Pol II promoter (CMV) and a minimal poly (A) cassette.

3. IMG-800 plasmid information

IMG-800 is a plasmid-based system to generate siRNAs. Inserts cloned into the vector express RNAs under U6 promoter in transfected mammalian cells. The functional neomycin resistance gene allows generation of permanent gene suppressed cell lines using G418 selection.

4. IMG-1200-2 plasmid information

pacAd5 is required for production of adenovirus in HEK-293 cells by homologous recombination with the shuttle plasmid.

5. IMK-515 Plasmid Sequence

 Schematic map and sequences of pCMV/SEAP and pNF-kB/SEAP plasmid vectors which are included in IMK-515 NF-kB SEAPorter™ Assay Kit.

6. RetroMax Retroviral System (Cat. No 10040K) Manual and Sequences

RetroMax retrovirus vector system is based on the pCL vector system developed by Naviaux et al. Included are sequences for the following vectors: pCLXSN (10041P), pCLNCX (10042P), pCLNRX (10043P), and pCLNDX (10044P).

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