Please contact your local IMGENEX distributor for pricing on these products. For a list of distributors, click here or go to www.imgenex.com/distributors.php
Application
Multiple: Please see individual data sheets for details Storage Store at 4°C, stable for 6 months. For long-term storage, store at -20°C.
Background
The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLR3, 7, 8, and 9 are expressed in the intracellular organelles. TLR7 and TLR8 have a common ligand, viral single-stranded RNA. TLR3 is activated by viral double-stranded RNA. TLR9 responds to bacterial unmethylated CpG DNA. Ten human homologs of TLRs (TLR1-10) have been described. Following are the different antibodies included in the TLR Sampler Kit:
Intracellular flow analysis of TLR7 in human PBMCs using IMG-581A at 0.5 ug/ml. Shaded histogram is cells alone, green is rabbit IgG isotype control (Imgenex, Cat. No. 20304), red represents anti-TLR7 antibody. Imgenex's goat anti-rabbit PE (IMGENEX, 20303) was used for secondary.
Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and human intestine tissue lysate (lane C) using IMG-315A at 2 ug/ml.
Intracellular flow analysis of TLR9 in Ramos cells using 0.1 ug of IMG-305A. Shaded histogram represents Ramos cells without antibody; green represents isotype control (IMGENEX, 20109); red represents anti-TLR9 antibody. Imgenex's goat anti-mouse FITC (IMGENEX, 20102) was used for secondary.
ffsdf
Related Products
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
Related Products
Related Product(s) of the Catalog # IMG-6318k
No related products found.
FAQ
Welcome to the IMGENEX Knowledgebase Center!
The IMGENEX Knowledgebase Center is an interactive tool containing hundreds of answers to frequently
asked technical questions compiled by IMGENEX technical support scientists. Updated daily, you can quickly
find information about specific products and applications.
